FAB2238P-025

antibody from R&D Systems

Targeting: KIR2DL4 103AS, 15.212, CD158D

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

FAB2238P-025

Provider

R&D Systems

Product name

Human KIR2DL4/CD158d PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Stains human KIR2DL4 transfected BaF/3 cells. It does not recognize transfectants that express KIR2DL1, 2DL2, 2DL3, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.

Reactivity

Human

Host

Mouse

Antigen sequence

Q99706


Isotype

IgG

Antibody clone number

181703

Vial size

25 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Human Leukocyte Antigen-G Inhibits the Anti-Tumor Effect of Natural Killer Cells via Immunoglobulin-Like Transcript 2 in Gastric Cancer.

Wan R, Wang ZW, Li H, Peng XD, Liu GY, Ou JM, Cheng AQ
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(5):1828-1841

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Antibody-Dependent NK Cell Activation Differentially Targets EBV-Infected Cells in Lytic Cycle and Bystander B Lymphocytes Bound to Viral Antigen-Containing Particles.

López-Montañés M, Alari-Pahissa E, Sintes J, Martínez-Rodríguez JE, Muntasell A, López-Botet M
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):656-665

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Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus.

Patel D, Gorelik G, Richardson B
Lupus (Los Angeles) 2016 Dec;1(3)

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HLA-G inhibition of NK-cell cytolytic function is uncoupled from tumor cell lipid raft reorganization.

Baudhuin J, Lesport E, Sousa S, Migraine J, Vigneron J, Lemaoult J, Carosella ED, Mooney N, Favier B
European journal of immunology 2012 Mar;42(3):700-9

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Decreased DNA methyltransferase levels contribute to abnormal gene expression in "senescent" CD4(+)CD28(-) T cells.

Liu Y, Chen Y, Richardson B
Clinical immunology (Orlando, Fla.) 2009 Aug;132(2):257-65

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Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells.

Li G, Weyand CM, Goronzy JJ
Journal of leukocyte biology 2008 Sep;84(3):824-34

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Functional impairment in circulating and intrahepatic NK cells and relative mechanism in hepatocellular carcinoma patients.

Cai L, Zhang Z, Zhou L, Wang H, Fu J, Zhang S, Shi M, Zhang H, Yang Y, Wu H, Tien P, Wang FS
Clinical immunology (Orlando, Fla.) 2008 Dec;129(3):428-37

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of KIR2DL4/CD158d in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 150 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days were stained with Mouse Anti-Human KIR2DL4/CD158d PE-conjugated Monoclonal Antibody (Catalog # FAB2238P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.