STJ110668

antibody from St John's Laboratory

Targeting: METTL3 M6A, MT-A70, Spo8

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: STJ110668 - Provider product page
Provider: St John's Laboratory
Product name: Anti-METTL3 antibody (1-156) (STJ110668)
Antibody type: Polyclonal
Description: Rabbit polyclonal antibody anti-METTL3 (1-156) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Handling: NA

METTL3 protein structure - STJ110668 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of HT-1080 cells, using METTL3 antibody (STJ110668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of various cell lines, using METTL3 antibody (STJ110668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of Rat spleen, using METTL3 antibody (STJ110668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Confocal immunofluorescence analysis of Hela cells using METTL3 Polyclonal Antibody (STJ110668) at dilution of 1:200. Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of U-2 OS cells using METTL3 rabbit polyclonal antibody (STJ110668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human esophageal cancer using METTL3 rabbit polyclonal antibody (STJ110668) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA