NB100-77311

antibody from Novus Biologicals

Targeting: METTL3 M6A, MT-A70, Spo8

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-77311

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-77311, RRID:AB_1085116

Product name

Rabbit Polyclonal Methyltransferase like 3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311] - Detection of Human METTL3 by Western Blot of immunoprecipitates. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-METTL3/MT-A70 antibody (NB100-77311) used for IP at 3 mcg/mg lysate. METTL3/MT-A70 was also immunoprecipitated by rabbit anti-METTL3/MT-A70 antibody (NB100-77310), which recognizes an upstream epitope. For blotting immunoprecipitated METTL3/MT-A70, NB100-77310 was used at 0.1 mcg/ml, and the ReliaBLOT(R) Reagents and Procedures (Bethyl Cat. No. WB120) were incorporated. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).anti-METTL3/MT-A70 antibody NB100-77310, which recognizes an upstream epitope.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- METTL3/MT-A70 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB