Explore
Validate
LearnANR-187-200UL
antibody from Invitrogen Antibodies
Targeting: TSPAN7
A15, CD231, DXS1692E, MRX58, MXS1, TALLA-1, TM4SF2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ANR-187-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tetraspanin 7 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:500). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody , preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Tetraspanin 7 in the rat substantia nigra region. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Tetraspanin 7 immunoreactivity (green) appears in neuronal profiles (arrows) in both pars reticulata (SNR) and pars compacta (SNC). B. Pre-incubation of the Antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Tetraspanin 7 in the mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region shows Tetraspanin 7 immunoreactivity (green) in neuronal profiles in the pyramidal (P) and stratum radiatum (SR, horizontal arrows) layers. Glial profiles appeared mainly in the stratum radiatum layer (vertical arrows). B. Pre-incubation of the Antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Tetraspanin 7 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (2.5 µg) + goat- Anti-rabbit-FITC.
