PA5-46070

antibody from Invitrogen Antibodies

Targeting: TTLL11 bA244O19.1, C9orf20

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46070

Provider

Invitrogen Antibodies

Product name

TTLL11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: QVLQRPPPTL PPSKPKPVQG LCPHGKPRDK GRSCKRSSGH GSGENGSQRP Sequence homology: Human: 100%; Rabbit: 92%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Genetic variant of TTLL11 gene and subsequent ciliary defects are associated with idiopathic scoliosis in a 5-generation UK family.

Mathieu H, Patten SA, Aragon-Martin JA, Ocaka L, Simpson M, Child A, Moldovan F
Scientific reports 2021 May 26;11(1):11026

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 WT and mutant TTLL11 protein expression after starvation. ( A ) WT (DE0194) and mutant (DE0193) fibroblasts were treated with medium without FBS for 24 h. Cells were stained with immunofluorescence for acetylated a alpha -Tubulin (red), TTLL11 (green) and DAPI (blue). Scale bar 20 mum. ( B ) Graphical representation of the percentage of TTLL11 mutant ciliated fibroblasts compared to WT fibroblasts (DE0194) n = 660. ( C ) Graphical representation of mean cilia length for TTLL11 mutant ciliated fibroblasts compared to WT fibroblasts (left panel) and distribution of ciliated cells based on cilia length: < 3 m mu m; 3-5 m mu m; > 5 m mu m (right panel); n = 185. Values shown as mean +- SD, the difference with controls was examined by unpaired, two-tailed Student's t-test, *P