PA5-37088

antibody from Invitrogen Antibodies

Targeting: CYP8B1 CYP12

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-37088

Provider

Invitrogen Antibodies

Product name

CYP8B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 58 kDa.

Concentration

1 mg/mL

References

Submitted references

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo.

Nikolaou N, Arvaniti A, Appanna N, Sharp A, Hughes BA, Digweed D, Whitaker MJ, Ross R, Arlt W, Penning TM, Morris K, George S, Keevil BG, Hodson L, Gathercole LL, Tomlinson JW
The Journal of endocrinology 2020 May;245(2):207-218

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP8B1 using CYP8B1 polyclonal antibody (Product # PA5-37088) at a dilution of 1:500. Lane 1: HEK293T cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: PC12 cell lysate.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 GCs regulate AKR1D1 expression through GR activation. Dexamethasone treatment decreases AKR1D1 mRNA (A) and protein expression (B). Addition of the glucocorticoid receptor antagonist RU486 (5 muM, 24 h) in the dexamethasone-treated HepG2 cells normalises the expression levels of AKR1D1 (A and B). RU486 also normalises the dexamethasone-induced expression of CYP8B1 (C and D). Representative Western blot images are shown, and formal quantification was performed in n = 5 replicates. Representative Western blot images are shown, and formal quantification was performed in n = 5 replicates. qPCR data were normalised to 18SrRNA. Data are presented as mean+- s.e. of n = 5 experiments, performed in triplicate, * P < 0.05, ** P < 0.01, *** P < 0.001, compared to vehicle-treated controls.