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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81025 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81025, RRID:AB_11176409
- Product name
- CYP8B1 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Cellular Accumulation and Toxic Effects of Bile Acids in Cyclosporine A-Treated HepaRG Hepatocytes.
Sharanek A, Burban A, Humbert L, Bachour-El Azzi P, Felix-Gomes N, Rainteau D, Guillouzo A
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):573-87
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):573-87
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of CYP8B1 Antibody (C-term) (GTX81025) in K562 cell line lysates (35ug/lane). CYP8B1 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody (C-term)(GTX81025), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CYP8B1 Antibody (C-term) (GTX81025) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
