Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX129298 - Provider product page
- Provider
- GeneTex
- Product name
- Cullin 4b antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Human DNA Ligase I Interacts with and Is Targeted for Degradation by the DCAF7 Specificity Factor of the Cul4-DDB1 Ubiquitin Ligase Complex.
Peng Z, Liao Z, Matsumoto Y, Yang A, Tomkinson AE
The Journal of biological chemistry 2016 Oct 14;291(42):21893-21902
The Journal of biological chemistry 2016 Oct 14;291(42):21893-21902
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg Neuro2A whole cell lysate/extract B. 30 £gg C8D30 whole cell lysate/extract C. 30 £gg NIH-3T3 whole cell lysate/extract D. 30 £gg Raw 264.7 whole cell lysate/extract E. 30 £gg C2Cl2 whole cell lysate/extract5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg PC-12 whole cell lysate/extract B. 30 £gg Rat2 whole cell lysate/extract5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg A375 whole cell lysate/extract 5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cullin 4b antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cullin 4b protein stained by Cullin 4b antibody (GTX129298) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cullin 4b antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. Cullin 4b antibody (GTX129298) dilution: 1:1000.