Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Other assay [1]
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Validation data
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- Product number
- 12916-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CUL4B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MMSQSSGSG DGNDDEATTS KDGGFSSPSP SAAAAAQEVR SATDGNTSTT PPTSAKKRKL NSSSSSSSNS SNEREDFDST SSSSSTPPLQ PRDSASPSTS SFCLGVSVAA SSHVPIQKKL RFEDTLEFVG FDAKMAEESS SSSSSSSPTA ATSQQQQLKN KSILISSVAS VHHANGLAKS STTVSSFANS KPGSAKKLVI KNFKDKPKLP ENYTDETWQK LKEAVEAIQN STSIKYNLEE LYQAVENLCS YKISANLYKQ LRQICEDHIK AQIHQFREDS LDSVLFLKKI DRCWQNHCRQ MIMIRSIFLF LDRTYVLQNS MLPSIWDMGL ELFRAHIISD QKVQNKTIDG IL (1-351 aa encoded by BC036216)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12916-1-AP ( CUL4B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of CUL4B antibody (12916-1-AP, 1:2,000) with si-Control and si-CUL4B transfected HeLa cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12916-1-AP (CUL4B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 12916-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (Product # 12916-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 12916-1-AP (CUL4B Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 12916-1-AP (CUL4B Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 12916-1-AP (CUL4B Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12916-1-AP ( CUL4B antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12916-1-AP ( CUL4B antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.