PA5-148245

antibody from Invitrogen Antibodies

Targeting: PITPNA PITPN, VIB1A

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-148245

Provider

Invitrogen Antibodies

Product name

PITPN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

100 µg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PITPN in U2OS cells. Samples were incubated with PITPN polyclonal antibody (Product # PA5-148245) using a dilution of 1:200 at 4°C overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PITPN in Molt-4 Whole Cell Lysate. Samples were incubated with PITPN polyclonal antibody (Product # PA5-148245) using a dilution of 1:100 followed by Goat Anti-Rabbit IgG (H+L)/HRP secondary antibody at a dilution of 1:10,000. Lane A: 0.5 mg Molt-4 Whole Cell Lysate. 4 µL anti- PITPNA rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa; Observed band size: 34 kDa.