A305-605A-T

antibody from Invitrogen Antibodies

Targeting: EMC1 KIAA0090

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-605A-T

Provider

Invitrogen Antibodies

Product name

EMC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse EMC1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, A-549, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EMC1 antibody (Product # A305-605A-T; lot A305-605A-T-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EMC1 antibody A305-605A-M (lot A305-605A-M-1) used for IP at 20 µl per reaction. EMC1 was also immunoprecipitated by rabbit anti-EMC1 antibodies A305-604A-M and BL22263. For blotting immunoprecipitated EMC1, A305-605A-M was used at 1:250. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse EMC1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EMC1 antibody A305-605A-M (lot A305-605A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-EMC1 antibody (Product # A305-605A-T (lot A305-605A-T-2) used for IP at 40 µL per reaction. EMC1 was also immunoprecipitated by a previous lot of this antibody (lot A305-605A-T-1). For blotting immunoprecipitated EMC1, Product # A305-605A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.