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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54956 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Steroid 21-hydroxylase Antibody / CYP21A2
- Antibody type
- Polyclonal
- Description
- This highly specific Steroid 21-hydroxylase antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Steroid 21-hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human MDA-MB-435 cell lysate with Steroid 21-hydroxylase antibody. Expected molecular weight: 52-56 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human HEK293 cells with Steroid 21-hydroxylase antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast carcinoma tissue with Steroid 21-hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human MDA-MB-231 cells with Steroid 21-hydroxylase antibody; Blue=isotype control, Green= Steroid 21-hydroxylase antibody.
