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LearnPA5-26003
antibody from Invitrogen Antibodies
Targeting: CYP21A2
CA21H, CAH1, CPS1, CYP21, CYP21B, P450c21B
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-26003 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP21A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 293 cells using a CYP21A2 polyclonal antibody (Product # PA5-26003) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CYP21A2 in 293 cells. Samples were incubated in CYP21A2 polyclonal antibody (Product # PA5-26003) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP21A2 in formalin-fixed and paraffin-embedded human breast carcinoma. Samples were incubated with CYP21A2 polyclonal antibody (Product # PA5-26003) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MDA-MB231 cells using a CYP21A2 polyclonal antibody (Product # PA5-26003) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CYP21A2 in MDA-MB231 cells (bottom histogram). Samples were incubated with CYP21A2 polyclonal antibody (Product # PA5-26003) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
