PA5-88211

antibody from Invitrogen Antibodies

Targeting: RPS18 D6S218E, HKE3, KE-3, KE3, S18

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88211

Provider

Invitrogen Antibodies

Product name

RPS18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKDV KDGKYSQVLA NGLDNKLRED LERLKKIRAH RGLRHFWGLR VRGQHTKTTG RRGRTVGVSK KK; Positive Samples: U-87MG, HT-29, LO2, SKOV3, A375, Mouse brain, Mouse kidney; Cellular Location: Cytoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.67 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPS18 in HepG2 cells. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPS18 in NIH/3T3 cells. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPS18 in PC-12 cells. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPS18 in THP-1 cells. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS18 in paraffin-embedded rat brain. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS18 in paraffin-embedded human thyroid cancer. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS18 in paraffin-embedded mouse kidney. Samples were incubated with RPS18 Polyclonal antibody (Product # PA5-88211) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.