PA5-31289

antibody from Invitrogen Antibodies

Targeting: GNA11 FBH, FBH2, FHH2, HHC2

Antibody data

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Validation data

GNA11 protein structure - PA5-31289 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GNA11 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GNA11 polyclonal antibody (Product # PA5-31289) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GNA11 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GNA11 Polyclonal Antibody (Product # PA5-31289) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using GNA11 Polyclonal Antibody (Product # PA5-31289). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. GNA11 Polyclonal Antibody (Product # PA5-31289) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GNA11 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GNA11 Polyclonal Antibody (Product # PA5-31289) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of GNA11 was achieved by transfecting MDA-MB-231 cells with GNA11 specific siRNAs (Silencer® select Product # s5862, s5863). Western blot analysis (Fig. a) was performed using whole cell extracts from the MDA-MB-231 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GNA11 Rabbit Polyclonal Antibody (Product # PA5-31289, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GNA11.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti- GNA11 Polyclonal Antibody, (Product # PA5-31289) and a ~42 kDa band corresponding to GNA11 was observed across the panel except for THP1. Few uncharacterized bands (* and **) were also observed in THP-1 and Mouse Lung respectively. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), MDA-MB-231 (Lane 2), MCF-7 (Lane 3), SH-SY5Y (Lane 4), PANC-1 (Lane 5), OVCAR-3 (Lane 6), THP-1 (Lane 7), tissue extracts (30 µg lysate) of Mouse Brain (Lane 8), Mouse Lung (Lane 9) and Rat Brain (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).