Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4021 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4021, RRID:AB_1578636
- Product name
- PFKFB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PFKFB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase.
Lange AJ, Pilkis SJ
Nucleic acids research 1990 Jun 25;18(12):3652
Nucleic acids research 1990 Jun 25;18(12):3652
Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase.
Algaier J, Uyeda K
Biochemical and biophysical research communications 1988 May 31;153(1):328-33
Biochemical and biophysical research communications 1988 May 31;153(1):328-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PFKFB1 polyclonal antibody (Cat # PAB4021) in Jurkat cell lysate (35 ug/lane). PFKFB1 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PFKFB1 polyclonal antibody (Cat # PAB4021) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)