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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29363 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PFKFB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, HeLa, Molt-4, Raji.
- Concentration
- 0.67 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PFKFB1 using 30 µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PFKFB1 polyclonal antibody (Product # PA5-29363) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PFKFB1 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PFKFB1 Polyclonal Antibody (Product # PA5-29363) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PFKFB1 in methanol-fixed HeLa cells using a PFKFB1 polyclonal antibody (Product # PA5-29363) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PFKFB1 (Product # PA5-29363) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
