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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-64080 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ASGRVQEAAR PEEVVSQTPL LRSRALVKRV TWNLQESESS APAEDRAPRA PLHRPQKPRE GAWDMEDVAP TGVRQAFSEL PF
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHRF1 was performed by loading HEK293T (lane 1), untreated (lane 2), non-targeted sgRNA (lane 3) and PHRF1 CRISPR KO (lane 4) cell lysates onto a polyacrylamide gel. Proteins were transferred to a membrane and blocked. Ponceau stained transfer of blot is shown to demonstrate similar loading. PHRF1 was detected using a PHRF1 polyclonal antibody (Product # PA5-64080). Chemiluminescent detection was performed. Data courtesy of Dr. Kanishk Jain at UNC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PHRF1 in human cell line A549 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PHRF1 Polyclonal Antibody (Product # PA5-64080).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHRF1 in human cerebellum tissue shows moderate nuclear and cytoplasmic positivity in Purkinje cells. Samples were probed using a PHRF1 Polyclonal Antibody (Product # PA5-64080).
