GTX102128

antibody from GeneTex

Targeting: POM121 DKFZP586G1822, DKFZP586P2220, KIAA0618, POM121A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX102128

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102128, RRID:AB_10732546

Product name

POM121 antibody [N2N3]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Tpr regulates the total number of nuclear pore complexes per cell nucleus.

McCloskey A, Ibarra A, Hetzer MW
Genes & development 2018 Oct 1;32(19-20):1321-1331

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Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import.

Rodriguez-Bravo V, Pippa R, Song WM, Carceles-Cordon M, Dominguez-Andres A, Fujiwara N, Woo J, Koh AP, Ertel A, Lokareddy RK, Cuesta-Dominguez A, Kim RS, Rodriguez-Fernandez I, Li P, Gordon R, Hirschfield H, Prats JM, Reddy EP, Fatatis A, Petrylak DP, Gomella L, Kelly WK, Lowe SW, Knudsen KE, Galsky MD, Cingolani G, Lujambio A, Hoshida Y, Domingo-Domenech J
Cell 2018 Aug 23;174(5):1200-1215.e20

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Evolution of a transcriptional regulator from a transmembrane nucleoporin.

Franks TM, Benner C, Narvaiza I, Marchetto MC, Young JM, Malik HS, Gage FH, Hetzer MW
Genes & development 2016 May 15;30(10):1155-71

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Proaggregant nuclear factor(s) trigger rapid formation of α-synuclein aggregates in apoptotic neurons.

Jiang P, Gan M, Yen SH, Moussaud S, McLean PJ, Dickson DW
Acta neuropathologica 2016 Jul;132(1):77-91

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Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin.

Schwartz M, Travesa A, Martell SW, Forbes DJ
Nucleus (Austin, Tex.) 2015;6(1):40-54

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The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function.

Chow KH, Elgort S, Dasso M, Powers MA, Ullman KS
Molecular biology of the cell 2014 Jan;25(1):160-8

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX102128 diluted at 1:2000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE GTX102128 diluted at 1:2000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] (GTX102128) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121(GTX102128) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11305 (Red) at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.