TA502595

antibody from OriGene

Targeting: ANKRD53 FLJ12056, FLJ36160

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502595

Provider

OriGene

Proper citation

OriGene Cat#TA502595, RRID:AB_11126687

Product name

ANKRD53 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Antibody type

Monoclonal

Description

ANKRD53 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Host

Mouse

Conjugate

Unconjugated

Epitope

ANKRD53

Isotype

IgG

Antibody clone number

OTI2E4

Vial size

100 µl

Concentration

0.63 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (RC206701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206701 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ANKRD53 antibody(TA502595), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-ANKRD53 antibody(TA502595),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC