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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-37865 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HIC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, rat, dog, pig and cow based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Hic-1 in (A) A549 and (B) Jurkat lysates was performed using 35 µg protein in RIPA buffer. Lysates were probed with a Hic-1 polyclonal antibody (Product # PA5-37865) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Hic-1 in HEK293 overexpressing human HIC1 using a HIC1 polyclonal antibody (Product # PA5-37865) at a dilution of 0.5 µg/mL. Lane 1 shows mock-transfected cells.
