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LearnPA5-112494
antibody from Invitrogen Antibodies
Targeting: H2BC5
H2B/b, H2BFB, HIST1H2BD
Western blot
ELISA
Immunocytochemistry Immunoprecipitation Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-112494 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H2BK23cr Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of H2BK23cr in Hela cells using a Polyclonal antibody (Product # PA5-112494) at a dilution of 1:37.5. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation analysis of H2BK23cr in 4*10^6 Hela cells using a H2BK23cr polyclonal antibody (Product #PA5-112494). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg anti-HIST1H2BC or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of H2BK23cr in K562 whole cell lysate using a Polyclonal antibody (Product #PA5-112494). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 1: Rabbit control IgG, Lane 2: 3 µg of primary antibody and 500 µg of K562 whole cell lysate, Lane 3: 20 µg of K562 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of H2BK23cr in K562 whole cell lysate using a Polyclonal antibody (Product #PA5-112494). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 1: Rabbit control IgG, Lane 2: 3 µg of primary antibody and 500 µg of K562 whole cell lysate, Lane 3: 20 µg of K562 whole cell lysate.
