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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-27611 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMIGO1 Monoclonal Antibody (L86/33)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-27611 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 60-80kDa depending on maturity/glycosylation. This antibody was formerly sold as clone S86-36.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- L86/33
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMIGO1 in rat brain membrane with 15 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with AMIGO1 monoclonal antibody (Product # MA5-27611) using a dilution of 1:200 (16 hours at RT), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:1000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder, Lane 2: Rat Brain membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AMIGO1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with AMIGO1 monoclonal antibody (Product # MA5-27611) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) AMIGO-1 Antibody, D) Merged image. Magnification: 60x.
