Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-22421 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Synaptotagmin 6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 55kDa. Does not cross-react with other synaptotagmins.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Synaptotagmin VI Antibody (S270-47) [NBP2-22421] - analysis of Rat brain lysates showing detection of Synaptotagmin 6 protein using Mouse Anti-Synaptotagmin 6 Monoclonal Antibody, Clone S270-47 . Primary Antibody: Mouse Anti-Synaptotagmin 6 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Synaptotagmin VI Antibody (S270-47) [NBP2-22421] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-6 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X.
