MA5-27599

antibody from Invitrogen Antibodies

Targeting: SYT6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27599

Provider

Invitrogen Antibodies

Product name

Anti-Synaptotagmin 6 Monoclonal Antibody (S270-47)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S270-47

Vial size

100 µg

Concentration

1 mg/ml

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Synaptotagmin 6 in rat brain lysates. The sample was incubated with Synaptotagmin 6 monoclonal antibody (Product # MA5-27599) using the following dilutions: 1:100, 1:250, 1:500 and 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Synaptotagmin 6 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Synaptotagmin 6 monoclonal antibody (Product # MA5-27599) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Synaptotagmin-6 Antibody, D) Merged image. Magnification: 60x.