PA5-120402

antibody from Invitrogen Antibodies

Targeting: METTL16 METT10D, MGC3329

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120402

Provider

Invitrogen Antibodies

Product name

METT10D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HeLa, A-549, NIH/3T3, C6, LNCaP, Rat testis. Immunogen sequence: RIQGVPKVTY TEFCQGRTMR WALAWSFYDD VTVPSPPSKR RKLEKPRKPI TFVVLASVMK ELSLKASPLR SETAEGIVVV TTWIEKILTD LKVQHKRVPC GKEEVSLFLT AIENSWIHLR RKKRERVRQL REVPRAPEDV IQALEEKKPT PKESGNSQEL ARGPQERTPC GPALREGEAA AVEGPCPSQE SLSQEENPEP TEDERSEEKG GVEVLESCQG SSNGAQDQEA SEQFGSPVAE RGKRLPGVAG QYLFKCLINV KKEVDDALVE MHWVEGQNRD LMNQLCTYIR NQIFRLVAVN

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.12 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of METT10D in C6 cells using a METT10D Polyclonal antibody (Product # PA5-120402). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of METT10D in C6 cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of METT10D in NIH/3T3 cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of METT10D in U-2 OS cells. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of METT10D in 300 μg extracts of LNCaP cells. Samples were precipitated with 3 μg METT10D Polyclonal antibody (Product # PA5-120402). Western blot was performed from the immunoprecipitate using METT10D Polyclonal antibody (Product # PA5-120402) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of METT10D in paraffin-embedded human spleen. Samples were incubated with METT10D Polyclonal antibody (Product # PA5-120402) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.