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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- F55026 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- FCGR1B Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific FCGR1B antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the FCGR1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) CCRF-CEM, 2) ThP-1 and 3) HeLa cell lysate with FCGR1B antibody. Predicted molecular weight: 22-32 kDa but may be observed at higher molecular weights due to glycosylation.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HL60, 2) HeLa and 3) K562 cell lysate with FCGR1B antibody. Predicted molecular weight: 22-32 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human appendix tissue with FCGR1B antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with FCGR1B antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human CCRF-CEM cells with FCGR1B antibody; Blue=isotype control, Green= FCGR1B antibody.
