Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-26205 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FCGR1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- Lot-specific
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FCGR1B in paraffin-embedded human appendix tissue. Samples were incubated with FCGR1B polyclonal antibody (Product # PA5-26205) using a dilution of 1:100 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FCGR1B in paraffin-embedded Human tonsil tissue. Samples were incubated with FCGR1B polyclonal antibody (Product # PA5-26205) using a dilution of 1:100 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using a FCGR1B polyclonal antibody (Product # PA5-26205) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FCGR1B in CEM cells (right histogram). Samples were incubated with FCGR1B polyclonal antibody (Product # PA5-26205) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).