AP8113a

antibody from Abgent

Targeting: NPR3 ANPRC, C5orf23, FLJ14054, GUCY2B, NPRC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8113a

Provider

Abgent

Proper citation

Abgent Cat#AP8113a, RRID:AB_2267530

Product name

Natriuretic Peptide Receptor C Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of ANPC-S82 (Cat. #AP8113a) in NCI-H460,HL-60 cell line lysates (35ug/lane). ANPC (arrow) was detected using the purified Pab."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic Peptide Receptor C (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus."

Primary Ab dilution

1:200

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) (Cat. #AP8113A)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50