Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-27515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calmodulin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calmodulin 2 using 50 µg of mouse brain lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Calmodulin 2 polyclonal antibody (Product # PA5-27515) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calmodulin 2 Polyclonal Antibody (Product # PA5-27515). Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin 2 Polyclonal Antibody (Product # PA5-27515) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Calmodulin 2 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 Polyclonal Antibody (Product # PA5 27515) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.