LS-B10601

antibody from LSBio

Targeting: AHSA1 Aha1, C14orf3, hAha1, p38

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B10601

Provider

LSBio

Product name

IHC-plus™ AHSA1 / AHA1 Antibody (clone 25F2.D9) LS-B10601

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Antibody clone number

25F2.D9

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-AHA1 Monoclonal Antibody - Western Blot. Western blot of anti-AHA1 monoclonal antibody shows detection of a band ~42 kD in size corresponding to AHA1 in A431 whole cell lysate (lane 1) and MCF-7 whole cell lysate (lane 2). A control lane is shown where primary ahntibody was omitted from the incubation (lane C). Molecular weight markers are shown at the left. For best results, block the membrane overnight with 3% BSA in TBS followed by reaction with primary antibody diluted 1:1000 and use HRP conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:20000 in blocking buffer (p/n MB-070) for detection.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-AHA1 Monoclonal Antibody - Immunohistochemistry. anti-AHA1 monoclonal antibody was used at a 5-10 ug/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. expression of AHA1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-AHA1 Monoclonal Antibody - Immunohistochemistry. anti-AHA1 monoclonal antibody was used at a 5-10 ug/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. expression of AHA1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.