Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX102312 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102312, RRID:AB_1949608
- Product name
- AHA-1 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 10% SDS PAGE GTX102312 diluted at 1:1000
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- GeneTex (provider)
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- Experimental details
- Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and AHA-1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A549, using AHA-1(GTX102312) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using AHA-1 (GTX102312) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AHA-1 antibody [N1C1] detects AHA-1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AHA-1 antibody [N1C1] (GTX102312) diluted at 1:500.