Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319551 - Provider product page
- Provider
- OriGene
- Product name
- Rat monoclonal anti-AHA1 antibody
- Antibody type
- Monoclonal
- Description
- Rat monoclonal anti-AHA1 antibody
- Host
- Rat
- Conjugate
- Unconjugated
- Epitope
- Ahsa1
- Antibody clone number
- 25F2.D9
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-AHA1 antibody shows detection of a band ~42 kDa corresponding to AHA1 in A431 whole cell lysate (lane 1) and MCF-7 whole cell lysate (lane 2). A control lane where primary ahntibody was omitted from the incubation (lane C). Molecular weight markers are shown at the left. For best results, block the membrane overnight with 3% BSA in TBS followed by reaction with primary antibody diluted 1:1,000 and use HRP conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AHA1 monoclonal antibody was used at a 5-10 ug/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
- Validation comment
- IHC
