H00010598-M02

antibody from Abnova Corporation

Targeting: AHSA1 Aha1, C14orf3, hAha1, p38

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010598-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010598-M02, RRID:AB_1112394

Product name

AHSA1 monoclonal antibody (M02), clone 4F3-B6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant AHSA1.

Antigen sequence

MAKWGEGDPRWIVEERANATNVNNWHWTERDASNW
STDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASI
NNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEI
PNLSGENSVDEVEISVSLAKDEPDTNLVALMKEEG
VKLLREAMGIYISTLKTEFTQGMILPTMNGESVDP
VGQPALKTEERKAKPAPSKTQARPVGVKIPTCKIT
LKETFLTSPEELYRVFTTQELVQAFTHAPATLEAD
RGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWP
EGHFATITLTFIDKNGETELCMEGRGIPAPEEERT
RQGWQRYYFEGIKQTFGYGARLF


Isotype

IgG

Antibody clone number

4F3-B6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of AHSA1 expression in transfected 293T cell line by AHSA1 monoclonal antibody (M02), clone 4F3-B6.Lane 1: AHSA1 transfected lysate (Predicted MW: 38.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AHSA1 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of AHSA1 transfected lysate using anti-AHSA1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AHSA1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol