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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB9927 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9927, RRID:AB_1672097
- Product name
- AHSA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AHSA1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis.
Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery.
Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1.
Wang X, Venable J, LaPointe P, Hutt DM, Koulov AV, Coppinger J, Gurkan C, Kellner W, Matteson J, Plutner H, Riordan JR, Kelly JW, Yates JR 3rd, Balch WE
Cell 2006 Nov 17;127(4):803-15
Cell 2006 Nov 17;127(4):803-15
Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery.
Meyer P, Prodromou C, Liao C, Hu B, Roe SM, Vaughan CK, Vlasic I, Panaretou B, Piper PW, Pearl LH
The EMBO journal 2004 Mar 24;23(6):1402-10
The EMBO journal 2004 Mar 24;23(6):1402-10
Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1.
Panaretou B, Siligardi G, Meyer P, Maloney A, Sullivan JK, Singh S, Millson SH, Clarke PA, Naaby-Hansen S, Stein R, Cramer R, Mollapour M, Workman P, Piper PW, Pearl LH, Prodromou C
Molecular cell 2002 Dec;10(6):1307-18
Molecular cell 2002 Dec;10(6):1307-18
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using AHSA1 polyclonal antibody (Cat # PAB9927) shows detection of AHSA1 in COS-7 cells.For Lanes 2 and 4, COS-7 cells were transfected with pcDNA3-FLAG-AHSA1.For Lanes 1 and 3, COS-7 cells were not transfected.Extracts (40 ug per lane) were electrophoresed and transferred to nitrocellulose.The membrane was probed with anti-AHSA1 (lanes 1 and 2, 1 : 2,000 dilution) or anti-FLAG (lanes 3 and 4).The lower band seen in anti-AHSA1 blotting (arrowhead) is endogenous AHSA1.Personal Communication, Brad Scroggins, CCR-NCI, Bethesda, MD.
