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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-45233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AHA1 Monoclonal Antibody (25F2.D9), APC
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- 1 µg/mL of MA5-45233 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.|Detects approximately 38kDa. Can run up to 45kDa on SDS Page.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 25F2.D9
- Vial size
- 100 µg
- Concentration
- 1.2 mg/mL
- Storage
- 4°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45233) at 1:1,000, followed by FITC Goat Anti-Rat (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45233) at 1:1,000, followed by FITC Goat Anti-Rat (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45233) at 1:1,000, followed by FITC Goat Anti-Rat (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis using HeLa cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45233) at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45233) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis using HeLa cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45233) at 1:1,000.
