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Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- MA5-45234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AHA1 Monoclonal Antibody (25F2.D9), FITC
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- 1 µg/mL of MA5-45234 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.|Detects approximately 38kDa. Can run up to 45kDa on SDS Page.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 25F2.D9
- Vial size
- 100 µg
- Concentration
- 1.2 mg/mL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000, followed by FITC Goat Anti-Rat (green).
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000, followed by FITC Goat Anti-Rat (green).
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis using HeLa cells. Samples were incubated with Aha1 monoclonal antibody (Product # MA5-45234) at 1:1,000.
- Conjugate
- Green dye
