Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6858 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NGFRAP1/BEX3/NADE Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NGFRAP1/BEX3 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references BEX3 contributes to cisplatin chemoresistance in nasopharyngeal carcinoma.
Gao W, Li JZ, Chen SQ, Chu CY, Chan JY, Wong TS
Cancer medicine 2017 Feb;6(2):439-451
Cancer medicine 2017 Feb;6(2):439-451
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse NGFRAP1/BEX3 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGFRAP1/BEX3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- NGFRAP1/BEX3 in Human Prostate. NGFRAP1/BEX3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.