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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ6827 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- BEX3 Antibody / NGFRAP1 / NADE
- Antibody type
- Polyclonal
- Description
- This highly specific BEX3 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the BEX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HCCT, 2) human HCCP, 3) human MCF7, 4) human HeLa, 5) rat liver, 6) rat L6, 7) mouse liver and 8) mouse C2C12 cell lysate with BEX3 antibody. Predicted molecular weight ~13 kDa (monomer) but commonly observed at ~24 kDa. The dimer may be observed at 40-50 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human MCF7 cells with BEX3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BEX3 antibody.
