PA5-20076

antibody from Invitrogen Antibodies

Targeting: BEX3 Bex, DXS6984E, HGR74, NADE, NGFRAP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20076

Provider

Invitrogen Antibodies

Product name

NADE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Despite its predicted molecular weight, NADE often migrates at ~23kDa in SDS-PAGE. A suggested positive control is human brain tissue lysate.

Concentration

1 mg/mL

References

Submitted references

Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice.

Qin T, Yuan Z, Yu J, Fu X, Deng X, Fu Q, Ma Z, Ma S
Clinical and translational medicine 2020 Dec;10(8):e243

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human brain cell lysates using a NADE polyclonal antibody (Product # PA5-20076) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NADE in Human brain cell lysates with NADE Polyclonal Antibody (Product # PA5-20076) at 1 µg/mL in the presence (A) or absence (B) of blocking peptide.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of NADE in human brain tissue with NADE Polyclonal Antibody (Product # PA5-20076) at 2 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

2 FIGURE SSd activated NRAGE/NADE/NRIF cell apoptotic signaling in the hippocampus. A, A heat map of the NRAGE pathway-related genes shows a strong correlation in SSd-treated mice (n = 5). B, GSEA profiles of the correlation between SSd-treated mice and NRAGE signaling. C, A heat map of the NRAGE/NRIF/NADE pathway-related genes shows a strong correlation in SSd-treated mice (n = 5). D, GSEA profiles of the correlation between SSd-treated mice and NRAGE/NRIF/NADE signaling. The upper part plots the enrichment scores for each gene, and the lower part of the plot shows the value of the ranking metric moving down the list of ranked genes. E, Western blot analysis of NRAGE/NRIF/NADE signaling in the hippocampus and quantitative analysis of the levels of NRAGE, NRIF, NADE, and p-JNK (n = 4). F, Hippocampal cell apoptosis was evaluated by TUNEL staining in the DG (n = 4). Scale bar 50 mum. G, Western blot analysis of the apoptotic proteins in the hippocampus and quantitative analysis of the levels of BAX, BCL2, cleaved caspase-3 (n = 4). Data are expressed as mean +- SEM. # P < .05 versus the control. ## P < .01 versus the control