PA5-72054

antibody from Invitrogen Antibodies

Targeting: RUBCN KIAA0226, rubicon, rundataxin

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72054

Provider

Invitrogen Antibodies

Product name

Rubicon Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BARON in U251 cells. Samples were treated with Chloroquine (50 µM,16h), then fixed with 4% paraformaldehyde (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PA5-72054 BARON (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of BARON in MDA-231 cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with BARON polyclonal antibody (Product # PA5-72054) followed by a FITC conjugated goat anti-rabbit secondary antibody.