F54720

antibody from NSJ Bioreagents

Targeting: RUBCN KIAA0226, rubicon, rundataxin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54720

Provider

NSJ Bioreagents

Product name

Rubicon Antibody / RUBCN / BARON

Antibody type

Polyclonal

Description

This highly specific Rubicon antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Immunofluorescence applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Rubicon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) K562 and 2) HEK293 cell lysate with Rubicon antibody. Expected molecular weight ~130 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with Rubicon antibody (green) and Hoechst 33342 nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lymph tissue with Rubicon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-231 cells with Rubicon antibody; Blue=isotype control, Green= Rubicon antibody.