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- References [26]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 21444-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#21444-1-AP, RRID:AB_11182941
- Product name
- Rubicon antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Rubicon antibody (Cat. #21444-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references p62 limits Salmonella Typhimurium in macrophages through its role in cell signalling.
RUBCN as a novel prognostic biomarker and therapeutic target in breast cancer.
Aspergillus fumigatus Gliotoxin Inhibits LC3-Associated Phagocytosis in Macrophages in a Calcium-Dependent Manner.
CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy.
Apoptotic breast cancer cells after chemotherapy induce pro-tumour extracellular vesicles via LAP-competent macrophages.
The marine-derived compound TAG alleviates Parkinson's disease by restoring RUBCN-mediated lipid metabolism homeostasis.
Agomelatine promotes differentiation of oligodendrocyte precursor cells and preserves white matter integrity after cerebral ischemic stroke.
All-trans retinoic acid induces lipophagy by reducing Rubicon in Hepa1c1c7 cells.
Autophagy and LC3-associated phagocytosis contribute negatively to the killing capability of THP-1-derived macrophages against Candida albicans at the mid-stage.
P38-DAPK1 axis regulated LC3-associated phagocytosis (LAP) of microglia in an in vitro subarachnoid hemorrhage model.
Inhibition of interaction between ROCK1 and Rubicon restores autophagy in endothelial cells and attenuates brain injury after prolonged ischemia.
Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.
Rubicon can predict prognosis in patients with pancreatic ductal adenocarcinoma after neoadjuvant chemoradiotherapy.
FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.
Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.
Rubicon-regulated beta-1 adrenergic receptor recycling protects the heart from pressure overload.
Dickkopf1 (Dkk1) Alleviates Vascular Calcification by Regulating the Degradation of Phospholipase D1 (PLD1).
Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.
17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis.
ZKSCAN3 in severe bacterial lung infection and sepsis-induced immunosuppression.
STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.
A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.
The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.
Inducible Rubicon facilitates viral replication by antagonizing interferon production.
USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.
Underwood D, Balci A, Solano-Collado V, Wilson HM, Baldassarre M, Spanò S
Access microbiology 2026;8(1)
Access microbiology 2026;8(1)
RUBCN as a novel prognostic biomarker and therapeutic target in breast cancer.
Yang DD, Liu CH, Jia SQ, Xue ZK, Zhang MM, Yang R, Huang YZ, Zhao XC, Han BS, Nie SD, Huang GL, Hou JX
PloS one 2026;21(1):e0341357
PloS one 2026;21(1):e0341357
Aspergillus fumigatus Gliotoxin Inhibits LC3-Associated Phagocytosis in Macrophages in a Calcium-Dependent Manner.
Sun Y, Chen F, Zhang L, Zheng C, Wang Y, Zhang Y, Han L
Journal of immunology research 2026;2026(1):e5542735
Journal of immunology research 2026;2026(1):e5542735
CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy.
Gao X, Yu Y, Sun J, Zhao H, You Y, Shi X, Wang K, Hong S, Xiong X, Huang C, Zhang H, Jiang G
PLoS biology 2026 Feb;24(2):e3003647
PLoS biology 2026 Feb;24(2):e3003647
Apoptotic breast cancer cells after chemotherapy induce pro-tumour extracellular vesicles via LAP-competent macrophages.
Zhang Q, Liu X, Wei Q, Xiong S, Luo W, Zhou Y, Cao J, Xu X, Liu R, Tang X, Zhang W, Luo B
Redox biology 2025 Mar;80:103485
Redox biology 2025 Mar;80:103485
The marine-derived compound TAG alleviates Parkinson's disease by restoring RUBCN-mediated lipid metabolism homeostasis.
Yang P, Liu Y, Tong ZW, Huang QH, Xie XH, Mao SY, Ding JH, Lu M, Tan RX, Hu G
Acta pharmacologica Sinica 2024 Jul;45(7):1366-1380
Acta pharmacologica Sinica 2024 Jul;45(7):1366-1380
Agomelatine promotes differentiation of oligodendrocyte precursor cells and preserves white matter integrity after cerebral ischemic stroke.
Wang S, Li C, Kang X, Su X, Liu Y, Wang Y, Liu S, Deng X, Huang H, Li T, Lu D, Cai W, Lu Z, Wei L, Lu T
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2024 Dec;44(12):1487-1500
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2024 Dec;44(12):1487-1500
All-trans retinoic acid induces lipophagy by reducing Rubicon in Hepa1c1c7 cells.
Nguyen AT, Masuda M, Mori Y, Adachi Y, Fukuda T, Furuichi A, Takikawa M, Tsuda Y, Hamada Y, Maruyama Y, Ohminami H, Ohnishi K, Taketani Y
Journal of lipid research 2024 Aug;65(8):100598
Journal of lipid research 2024 Aug;65(8):100598
Autophagy and LC3-associated phagocytosis contribute negatively to the killing capability of THP-1-derived macrophages against Candida albicans at the mid-stage.
Li D, Wang L, Zhao Z, Bai C, Li X
Immunology letters 2023 Nov;263:25-32
Immunology letters 2023 Nov;263:25-32
P38-DAPK1 axis regulated LC3-associated phagocytosis (LAP) of microglia in an in vitro subarachnoid hemorrhage model.
Chen XX, Tao T, Liu XZ, Wu W, Wang JW, Yue TT, Li XJ, Zhou Y, Gao S, Sheng B, Peng Z, Xu HJ, Ding PF, Wu LY, Zhang DD, Lu Y, Hang CH, Li W
Cell communication and signaling : CCS 2023 Jul 21;21(1):175
Cell communication and signaling : CCS 2023 Jul 21;21(1):175
Inhibition of interaction between ROCK1 and Rubicon restores autophagy in endothelial cells and attenuates brain injury after prolonged ischemia.
Lin Y, Zhan Z, Hu M, Li H, Zhang B, Wu R, Tan S, Shan Y, Lu Z, Qin B
Journal of neurochemistry 2023 Jan;164(2):172-192
Journal of neurochemistry 2023 Jan;164(2):172-192
Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.
Li T, Su X, Lu P, Kang X, Hu M, Li C, Wang S, Lu D, Shen S, Huang H, Liu Y, Deng X, Cai W, Wei L, Lu Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Aug;10(22):e2206432
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Aug;10(22):e2206432
Rubicon can predict prognosis in patients with pancreatic ductal adenocarcinoma after neoadjuvant chemoradiotherapy.
Marukawa D, Gotoh K, Kobayashi S, Sasaki K, Iwagami Y, Yamada D, Tomimaru Y, Akita H, Asaoka T, Noda T, Takahashi H, Tanemura M, Doki Y, Eguchi H
International journal of clinical oncology 2023 Apr;28(4):576-586
International journal of clinical oncology 2023 Apr;28(4):576-586
FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.
Cai W, Hu M, Li C, Wu R, Lu D, Xie C, Zhang W, Li T, Shen S, Huang H, Qiu W, Liu Q, Lu Y, Lu Z
Autophagy 2023 Apr;19(4):1144-1163
Autophagy 2023 Apr;19(4):1144-1163
Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.
Shen S, Cheng X, Zhou L, Zhao Y, Wang H, Zhang J, Sun X, Wang Y, Shu Y, Xu Y, Tao Y, Li M, Lu Z, Cai W, Nie G, Qiu W
ACS nano 2022 Nov 22;16(11):18886-18897
ACS nano 2022 Nov 22;16(11):18886-18897
Rubicon-regulated beta-1 adrenergic receptor recycling protects the heart from pressure overload.
Akazawa Y, Taneike M, Ueda H, Kitazume-Taneike R, Murakawa T, Sugihara R, Yorifuji H, Nishida H, Mine K, Hioki A, Omiya S, Nakayama H, Yamaguchi O, Yoshimori T, Sakata Y, Otsu K
Scientific reports 2022 Jan 7;12(1):41
Scientific reports 2022 Jan 7;12(1):41
Dickkopf1 (Dkk1) Alleviates Vascular Calcification by Regulating the Degradation of Phospholipase D1 (PLD1).
Li X, Liu XL, Li X, Zhao YC, Wang QQ, Zhong HY, Liu DD, Yuan C, Zheng TF, Zhang M
Journal of cardiovascular translational research 2022 Dec;15(6):1327-1339
Journal of cardiovascular translational research 2022 Dec;15(6):1327-1339
Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.
Zhou C, Dong X, Wang M, Qian X, Hu M, Liang K, Liang Y, Zhang R, Huang Y, Lyu H, Xiao S, Tang Y, Ali DW, Michalak M, Chen XZ, Tang J
Cell insight 2022 Aug;1(4):100045
Cell insight 2022 Aug;1(4):100045
17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis.
Sun Z, Qu J, Xia X, Pan Y, Liu X, Liang H, Dou H, Hou Y
International journal of biological sciences 2021;17(2):460-474
International journal of biological sciences 2021;17(2):460-474
ZKSCAN3 in severe bacterial lung infection and sepsis-induced immunosuppression.
Ouyang X, Becker E Jr, Bone NB, Johnson MS, Craver J, Zong WX, Darley-Usmar VM, Zmijewski JW, Zhang J
Laboratory investigation; a journal of technical methods and pathology 2021 Nov;101(11):1467-1474
Laboratory investigation; a journal of technical methods and pathology 2021 Nov;101(11):1467-1474
STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.
Zhou C, Qian X, Hu M, Zhang R, Liu N, Huang Y, Yang J, Zhang J, Bai H, Yang Y, Wang Y, Ali D, Michalak M, Chen XZ, Tang J
Autophagy 2020 Oct;16(10):1786-1806
Autophagy 2020 Oct;16(10):1786-1806
A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.
Sun P, Nie K, Zhu Y, Liu Y, Wu P, Liu Z, Du S, Fan H, Chen CH, Zhang R, Wang P, Cheng G
Nature communications 2020 Jan 14;11(1):260
Nature communications 2020 Jan 14;11(1):260
The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.
Huang X, Gan G, Wang X, Xu T, Xie W
Autophagy 2019 Jul;15(7):1258-1279
Autophagy 2019 Jul;15(7):1258-1279
RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.
Muniz-Feliciano L, Doggett TA, Zhou Z, Ferguson TA
Autophagy 2017;13(12):2072-2085
Autophagy 2017;13(12):2072-2085
Inducible Rubicon facilitates viral replication by antagonizing interferon production.
Wan Y, Cao W, Han T, Ren S, Feng J, Chen T, Wang J, Broering R, Lu M, Zhu Y
Cellular & molecular immunology 2017 Jul;14(7):607-620
Cellular & molecular immunology 2017 Jul;14(7):607-620
USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.
Xu D, Shan B, Sun H, Xiao J, Zhu K, Xie X, Li X, Liang W, Lu X, Qian L, Yuan J
Genes & development 2016 Aug 1;30(15):1718-30
Genes & development 2016 Aug 1;30(15):1718-30
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse liver tissue were subjected to SDS PAGE followed by western blot with 21444-1-AP(KIAA0226 antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 21444-1-AP(KIAA0226 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Rubicon antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human Rubicon. This antibody recognizes human,mouse,rat antigen. The Rubicon antibody has been validated for the following applications: ELISA, WB, IHC analysis.
