Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS3377 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3377, RRID:AB_1662166
- Product name
- FTÉÇ (E285) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 250-300 of Human FTÉÇ.
- Description
- Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical Ï« and ÉÇ subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an a-b heterodimer. The ÉÇ subunit, which is known as FTÉÇ, CAAX farnesyltransferase subunit ÉÇ, or Ras proteins prenyltransferase subunit ÉÇ, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate. The Ï« subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of FT¦Â (E285) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate