AP16535a

ZNF81 antibody from Abgent

HFZ20, MRX45

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Provider product page for AP16535a

Antibody Data

Product number

AP16535a

Provider

Abgent

Proper citation

Abgent Cat#AP16535a, RRID:AB_11140582

Product name

ZNF81 Antibody (N-term)

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZNF81 Antibody (N-term) (Cat. #AP16535a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ZNF81 antibody detected the ZNF81 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of U251 cell stained with ZNF81 Antibody (N-term)(Cat#AP16535a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF81 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ZNF81 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly."

Primary Ab dilution

1:10~50