PA5-95876

antibody from Invitrogen Antibodies

Targeting: TMEM147 MGC1936, NIFIE14

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95876

Provider

Invitrogen Antibodies

Product name

TMEM147 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: GLNLVMSRNA GKGEYK

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.35 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Substrate-driven assembly of a translocon for multipass membrane proteins.

Sundaram A, Yamsek M, Zhong F, Hooda Y, Hegde RS, Keenan RJ
Nature 2022 Nov;611(7934):167-172

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An ER translocon for multi-pass membrane protein biogenesis.

McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ
eLife 2020 Aug 21;9

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Natively isolated TMCO1-ribosome complexes contain multiple transmembrane components. ( A ) Emetine- and micrococcal nuclease-treated membranes from wild-type (WT) or 3xFlag-TMCO1 (Flag) HEK293 cells were digitonin-solubilized, immunoprecipitated via the 3xFlag tag on TMCO1, and the eluate sedimented through a sucrose cushion to isolate the ribosome-associated fraction for analysis. ( B ) Proteins enriched in the ribosomal fraction after immunoprecipitation from 3xFlag-TMCO1 or wild-type membranes. ( C ) Top hits were confirmed by western blotting. The catalytic STT3A subunit of the OST complex is not detected. ( D ) Topology and domain structure for the top hits, based on Uniprot annotation, homology modeling, de novo structure prediction (in RaptorX-Contact), and experimental mapping; the Sec61 complex is not shown. Distinguishing features include the large globular luminal domain of Nicalin (in contrast with the flexible luminal domains of NOMO and CCDC47), the large globular cytosolic domain of CCDC47 (with a conserved C-terminal coiled-coil), and a conserved cytosolic coiled-coil between the first two TMDs of TMCO1. TMEM147 is the core, multi-pass subunit of the Nicalin-TMEM147-NOMO complex ; note that the short extra-membrane loops of TMEM147 make it difficult to detect by mass spectrometry. Figure 1-figure supplement 1. Additional interaction analysis of the TMCO1 translocon components. ( A ) Comparison of co-purifying components in the ribosome-bound fractio