AF6479

antibody from Novus Biologicals

Targeting: BASP1 CAP-23, CAP23, NAP-22, NAP22

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6479

Provider

Novus Biologicals

Product name

Sheep Polyclonal BASP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human BASP1 in Western blots.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human BASP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BASP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6479) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BASP1 at approximately 60 kDa, possibly a higher molecular weight isoform or a product of SUMOyation. Lower molecular weight isoforms were also observed. This experiment was conducted under reducing conditions on a 4-20% SDS-PAGE gel and using Immunoblot Buffer Group 1.