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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF6479 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BASP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human BASP1 in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P80723- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human BASP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BASP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6479) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BASP1 at approximately 60 kDa, possibly a higher molecular weight isoform or a product of SUMOyation. Lower molecular weight isoforms were also observed. This experiment was conducted under reducing conditions on a 4-20% SDS-PAGE gel and using Immunoblot Buffer Group 1.
