HPA057058

antibody from Atlas Antibodies

Targeting: ONECUT2 OC-2

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA057058

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA057058, RRID:AB_2683323

Product name

Anti-ONECUT2

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ONECUT2, Gene description: one cut homeobox 2, Alternative Gene Names: OC-2, Validated applications: ICC, ChIP, Uniprot ID: O95948, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

ONECUT2 acts as a lineage plasticity driver in adenocarcinoma as well as neuroendocrine variants of prostate cancer

Qian C, Yang Q, Rotinen M, Huang R, Kim H, Gallent B, Yan Y, Cadaneanu R, Zhang B, Kaochar S, Freedland S, Posadas E, Ellis L, Di Vizio D, Morrissey C, Nelson P, Brady L, Murali R, Campbell M, Yang W, Knudsen B, Mostaghel E, Ye H, Garraway I, You S, Freeman M
Nucleic Acids Research 2024;52(13):7740-7760

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Qian C, Yang Q, Rotinen M, Huang R, Kim H, Gallent B, Yan Y, Cadaneanu R, Zhang B, Kaochar S, Freedland S, Posadas E, Ellis L, Di Vizio D, Morrissey C, Nelson P, Brady L, Murali R, Campbell M, Yang W, Knudsen B, Mostaghel E, Ye H, Garraway I, You S, Freeman M
2023

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ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY.

Liu D, Zhang T, Chen X, Zhang B, Wang Y, Xie M, Ji X, Sun M, Huang W, Xia L
Cell death & disease 2021 Nov 27;12(12):1113

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ONECUT2 overexpression promotes RAS-driven lung adenocarcinoma progression

Ma Q, Wu K, Li H, Li H, Zhu Y, Hu G, Hu L, Kong X
Scientific Reports 2019;9(1)

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ONECUT2 is a targetable master regulator of lethal prostate cancer that suppresses the androgen axis

Rotinen M, You S, Yang J, Coetzee S, Reis-Sobreiro M, Huang W, Huang F, Pan X, Yáñez A, Hazelett D, Chu C, Steadman K, Morrissey C, Nelson P, Corey E, Chung L, Freedland S, Di Vizio D, Garraway I, Murali R, Knudsen B, Freeman M
Nature Medicine 2018;24(12):1887-1898

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A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease.

Villaescusa JC, Li B, Toledo EM, Rivetti di Val Cervo P, Yang S, Stott SR, Kaiser K, Islam S, Gyllborg D, Laguna-Goya R, Landreh M, Lönnerberg P, Falk A, Bergman T, Barker RA, Linnarsson S, Selleri L, Arenas E
The EMBO journal 2016 Sep 15;35(18):1963-78

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-ONECUT2 (HPA057058, Lot 000044310) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.