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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-64172 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEZ6L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: RGLISDAQSL YVELLSETPA NPLLLSLRFE AFEEDRCFAP FLAHGNVTTT DPEYRPGALA TFSCLPGYAL EPPGPPNAIE CV Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Seizure-related 6 homolog like 2 autoimmunity: Neurologic syndrome and antibody effects.
Landa J, Guasp M, Petit-Pedrol M, Martínez-Hernández E, Planagumà J, Saiz A, Ruiz-García R, García-Fernández L, Verschuuren J, Saunders-Pullman R, Ramirez-Gómez L, Geschwind MD, Dalmau J, Sabater L, Graus F
Neurology(R) neuroimmunology & neuroinflammation 2021 Jan;8(1)
Neurology(R) neuroimmunology & neuroinflammation 2021 Jan;8(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SEZ6L2 in human cerebral cortex using SEZ6L2 Polyclonal Antibody (Product # PA5-64172) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SEZ6L2 in human cerebral cortex and tonsil tissues using SEZ6L2 Polyclonal Antibody (Product # PA5-64172). Corresponding SEZ6L2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SEZ6L2 in human tonsil using SEZ6L2 Polyclonal Antibody (Product # PA5-64172) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Detection of SEZ6L2-ab using a HEK293 cell-based assay HEK293 cells were transfected to express SEZ6L2 and incubated live, not permeabilized, with a patient's (A-C) or control (D-F) serum. Patient's serum, but not control serum, stained the cell surface of cells (green) that specifically express SEZ6L2, as demonstrated by the Myc-tag antibody (red). Both reactivities are shown merged in C. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI). Scale bar = 10 mum. (G, upper row): Western blot showing that the 4 patients' serum samples with SEZ6L2-ab (lanes 2-5) immunoprecipitated SEZ6L2. Lane 1 shows the immunoprecipitation using the commercial antibody, and lanes 6-9 demonstrate the lack of SEZ6L2 immunoprecipitation with 6 control samples. (G, lower row): Immunoblot strips of cell lysates of SEZ6L2-expressing HEK293 cells under denaturing conditions. None of the patient's serum (lanes 2-5) reacted with the denaturalized SEZ6L2 protein. Strips were also tested with a commercial SEZ6L2-ab (lane 1) and 6 control human sera (lanes 6-11). (H-K) Example of detection of SEZ6L2-ab IgG subclasses using a HEK293 cell-based assay. This patient's serum had IgG1 and IgG4 SEZ6L2-ab (green reactivity) being the IgG1 subclass immunoreactivity (H) more intense than that of the IgG4 (K). Nuclei counterstained with DAPI. IgG = immunoglobulin G.
